Increasing awareness of children’s developmental needs and rights has led to a global move toward giving children and adolescents a greater degree of autonomy in the decisions affecting their own lives. The present article presents two studies examining the role of culture and religion in twelve-year-old children’s perceptions of their rights across three cultures. The first study showed that U.S. and Swiss children advocated for more self-determination rights than Chinese-Malaysian children; U.S. and Chinese-Malaysian children advocated for more nurturance rights than Swiss children. Within the Chinese-Malaysian sample, Buddhist children were more likely to advocate for self-determination rights as compared to Christian children. Using a revised Children’s Rights Interview (rCRI), the second study showed that, on average U.S. and Chinese-Malaysian children were more likely to advocate for self-determination rights than nurturance rights. However, there were no significant differences among the two cultural groups in terms of the overall responses. The results of the studies are discussed in terms of the cultural orientation and constructivist frameworks.